Carbon monoxide fermentation to bioplastic: the effect of substrate adaptation on Rhodospirillum rubrum
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چکیده
منابع مشابه
Carbon monoxide-dependent growth of Rhodospirillum rubrum.
Under dark, anaerobic conditions in the presence of sufficient nickel, Rhodospirillum rubrum grows with a doubling time of under 5 h by coupling the oxidation of CO to the reduction of H+ to H2. CO-dependent growth of R. rubrum UR294, bearing a kanamycin resistance cassette in cooC, depends on a medium nickel level ninefold higher than that required for optimal growth of coo+ strains.
متن کاملCarbon monoxide dehydrogenase from Rhodospirillum rubrum.
The carbon monoxide dehydrogenase from the photosynthetic bacterium Rhodospirillum rubrum was purified over 600-fold by DEAE-cellulose chromatography, heat treatment, hydroxylapatite chromatography, and preparative scale gel electrophoresis. In vitro, this enzyme catalyzed a two-electron oxidation of CO to form CO2 as the product. The reaction was dependent on the addition of an electron accept...
متن کاملLife on carbon monoxide: X-ray structure of Rhodospirillum rubrum Ni-Fe-S carbon monoxide dehydrogenase.
A crystal structure of the anaerobic Ni-Fe-S carbon monoxide dehydrogenase (CODH) from Rhodospirillum rubrum has been determined to 2.8-A resolution. The CODH family, for which the R. rubrum enzyme is the prototype, catalyzes the biological oxidation of CO at an unusual Ni-Fe-S cluster called the C-cluster. The Ni-Fe-S C-cluster contains a mononuclear site and a four-metal cubane. Surprisingly,...
متن کاملThe effect of monofluoroacetate on the metabolism of Rhodospirillum rubrum.
The Athiorhodaceae are photosynthetic bacteria which grow anaerobically on media containing any one of a number of organic compounds, plus carbon dioxide and a source of growth factors, provided that the-cultures are illuminated. In contrast to the green plants, no oxygen is produced during photosynthesis. A number of strains will grow in the dark provided that oxygen is present. Themost genera...
متن کاملCarbon roadmap from syngas to polyhydroxyalkanoates in Rhodospirillum rubrum.
The gasification of organic waste materials to synthesis gas (syngas), followed by microbial fermentation, provides a significant resource for generating bioproducts such as polyhydroxyalkanoates (PHA). The anaerobic photosynthetic bacterium, Rhodospirillum rubrum, is an organism particularly attractive for the bioconversion of syngas into PHAs. In this study, a quantitative physiological analy...
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ژورنال
عنوان ژورنال: Biomass Conversion and Biorefinery
سال: 2020
ISSN: 2190-6815,2190-6823
DOI: 10.1007/s13399-020-00876-x